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15th February 2012 @ 03:17
KAB8-1

In IPA:Hex:TEA
40:60:0.1


KAB8-1_IPA40 flow rate 1 mL per minute

retention time 15 minutes, no separation

KAB8-1_IPA40 flow rate 0.5 mL per minute

retention time 27.6 and 29 minutes, some separation

KAB8-1_IPA40flow rate 0.35 mL per minute

retention time 52and 55 minutes, some separation but broad peaks

In IPA:Hex:TEA
80:20:0.1


KAB8-1_IPA80flow rate 0.35 mL per minute

retention time 34 and 39 minutes, some separation but not all the way to baseline

In IPA:Hex:TEA
90:10:0.1


KAB8-1_IPA90flow rate 0.35 mL per minute

retention time 37and 41 minutes, not as good as 80%

Conclusion, need to change polar solvent as IPA is too viscous and reduced flow rates lead to broadening of peaks.

In EtOH:Hex:TEA
50:50:0.1


KAB8-1_EtOH50 flow rate 1 mL per minute

retention time 11.7 and 12.8 minutes, some separation but too quick

KAB8-1_EtOH50 flow rate 0.5 mL per minute

retention time 24.1 and 26.2 minutes, some separation but not at baseline

In EtOH:Hex:TEA
25:75:0.1


KAB8-1_EtOH25 flow rate 1 mL per minute

retention time 21.9 and 24.5 minutes, some separation but just short of the baseline

KAB8-1_EtOH25 flow rate 0.5 mL per minute

retention time 45.9 and 51.4 minutes, baseline separation.
Attached Files
mnr_kab8-1_40IPA_1ml.pdf
mnr_kab8-1_40IPA_05ml.pdf
mnr_kab8-1_40IPA_035ml.pdf
mnr_kab8-1_80IPA.pdf
mnr_kab8-1_90IPA_035.pdf
mnr_kab8-1_25EtOH_1ml.pdf
mnr_kab8-1_25EtOH_05ml.pdf
mnr_kab8-1_50EtOH_1ml.pdf
mnr_kab8-1_50EtOH_05ml.pdf
MNR KAB8-1_40IPA_1mL.pdf
MNR KAB8-1_40IPA_05mL.pdf